A new enzymatic spectrophotometric assay for the determination of lactulose in milk
نویسندگان
چکیده
منابع مشابه
A new enzymatic spectrophotometric assay for the determination of lactulose in milk
A new enzymatic spectrophotometric assay for the determination of lactulose in milk samples was developed. This method entailed the use of b-galactosidase which hydrolyses lactulose giving fructose and galactose, and fructose dehydrogenase which reacts with fructose in presence of a tetrazolium salt (MTT), giving a coloured compound which can be detected spectrophotometrically at 570 nm. The as...
متن کاملNew Enzymatic Colorimetric Method for the Quantitative Determination of Phenylalanine in Dry-Blood Spots
Phenylketonuria (PKU) is an autosomal recessive disorder, which is characterized by severe mental retardation, microcephaly and seizures. The symptoms of this disease can be prevented if detected soon after birth. Therefore, blood Phenylalanine (Phe) measurement is essential for the early diagnosis, treatment and dietary monitoring of PKU patients. The goal of this research was to introduce a r...
متن کاملstudy of cohesive devices in the textbook of english for the students of apsychology by rastegarpour
this study investigates the cohesive devices used in the textbook of english for the students of psychology. the research questions and hypotheses in the present study are based on what frequency and distribution of grammatical and lexical cohesive devices are. then, to answer the questions all grammatical and lexical cohesive devices in reading comprehension passages from 6 units of 21units th...
a time-series analysis of the demand for life insurance in iran
با توجه به تجزیه و تحلیل داده ها ما دریافتیم که سطح درامد و تعداد نمایندگیها باتقاضای بیمه عمر رابطه مستقیم دارند و نرخ بهره و بار تکفل با تقاضای بیمه عمر رابطه عکس دارند
Rapid determination of lactulose in milk by microdialysis and biosensors.
A simple and rapid flow system for the determination of lactulose in milk samples was developed. It is based on the hydrolysis of lactulose to galactose and fructose by the enzyme beta-galactosidase immobilised in a reactor. The amount of fructose produced was measured with an electrochemical biosensor based on the fructose dehydrogenase enzyme, K3[Fe(CN)6] as mediator and a platinum based elec...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytica Chimica Acta
سال: 2000
ISSN: 0003-2670
DOI: 10.1016/s0003-2670(99)00765-5